MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients
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چکیده
منابع مشابه
MEKC of desmosine and isodesmosine in urine of chronic destructive lung disease patients.
Degradation of extracellular matrix components is central to many pathological features of chronic destructive lung disorders. Desmosine and isodesmosine are elastin-derived cross-linked amino acids whose urine levels are considered representative of elastin breakdown. The aim of this study was to apply a novel methodology, based on high-performance capillary electrophoresis, to the quantificat...
متن کاملThe biosynthesis of desmosine and isodesmosine.
The biosynthesis of desmosine and isodesmosine was studied with chick embryo aortas in tissue culture. Radioactivity was incorporated into desmosine and isodesmosine when uniformly labeled lysine-W was added to the medium, but no activity could be incorporated into these amino acids from aspartic acidJ4C, glutamic acidJ4C, or threonine-14C. The labeled desmosine and isodesmosine obtained after ...
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15 صفحه اولQuantitation of desmosine and isodesmosine in urine, plasma, and sputum by LC-MS/MS as biomarkers for elastin degradation.
The aim of this study is to develop a standardized LC-MS/MS method for accurate measurement of desmosine (DES) and isodesmosine (IDS) in all body fluids as biomarkers for in vivo degradation of matrix tissue elastin in man and animals. A reproducible three-step analytical procedure: (1) sample hydrolysis in 6N HCl, (2) SPE by a CF1 cartridge with addition of acetylated pyridinoline as internal ...
متن کاملThe detection and quantitation of free desmosine and isodesmosine in human urine and their peptide-bound forms in sputum.
Desmosine (D) and isodesmosine (I), the intramolecular crosslinking amino acids that occur in chains of elastin, have now been found in free form in human urine. Until now, these amino acids (M(r) = 526) were found to occur in urine only as higher molecular weight (M (r) = 1,000-1,500) peptides. Thus, the previously used analytical methods required, as the first step, acid hydrolysis of the uri...
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ژورنال
عنوان ژورنال: European Respiratory Journal
سال: 2000
ISSN: 0903-1936,1399-3003
DOI: 10.1034/j.1399-3003.2000.01511.x